RNA interference (RNAi) is a crucial technology for studying gene function in both in vitro and in vivo experiments and has also been utilized in developing RNAi-based therapies. Viral vectors, such as AAV, adenovirus, and lentivirus, are well-established systems for delivering RNAi through shRNA expression, offering high safety, efficiency, and specificity. At Ignis Bioscience, we provide a comprehensive range of shRNA services, including shRNA design, cloning, RNA interference efficiency screening and virus packaging (LV, AAV).

​

shRNA Design and Vector Construction
● shRNA sequences are designed based on the target gene and driven by pol III promoters (H1, U6).
● We offer inducible shRNA expression plasmids using the Cre-LoxP system for targeted gene interference.
● Our miR30-based shRNA plasmids, driven by pol II promoters (such as CMV, EF1a, CAG, TH, GFAP), enable specific gene silencing.

​

Guaranteed shRNA Knockdown
We provide three expression plasmids per target gene, ensuring that at least one achieves a knockdown effect of 70% or more on gene expression as verified by qRT-PCR. We will provide you the knock down efficiency report for your reference. 

​

Markers and Reporters
We offer vectors with mCherry or eGFP reporter genes for monitoring transfection or transduction efficiencies, with stable cell lines selected using a puromycin marker.

​

Delivery Formats
● Three individual shRNA constructs with 5 µg of purified plasmid.
● shRNA screening report.
● Verified lentivirus-shRNA or AAV-shRNA, along with a scrambled control virus.

​

Ignis Bioscience brings extensive expertise in RNAi technology and can tailor optimal experimental conditions to meet your needs. For inquiries or requests, please email us at support@ignisbio.com.